Calcineurin inhibitors block NFAT activation and protect patients from graft-versus-host disease during bone marrow transplantation. On the other hand, they exert adverse side effects and interfere with the valuable graft-versus-leukemia effect. In contrast, NFAT deficiency maintains graft-versus-leukemia activity, although still protecting from graft-versus-host disease in mouse models. Therefore, it is planned to evaluate new NFAT inhibitors in vitro, on engineered human skin and in mouse models, as well as to ablate NFAT family members by CRISPR/Cas9 ahead of cell transfer.
PD Dr. rer. nat Friederike Berberich-Siebelt | PD Dr. med. Silvia Spörl |
Universität Würzburg | University Hospital Erlangen |
Institut für Pathologie | Department of Medicine 5 |
Josef-Schneider-Straße 2 | Ulmenweg 18 |
97080 Würzburg | 91054 Erlangen |
T: 0931 31-81208 | T: +49 9131 8545021 |
Previously, we demonstrated in mice that targeting of TNFR2 and Fn14 allows GvL effect-sparing inhibition of GvHD by different mechanisms. Now, we will clarify whether co-targeting of TNFR2 and Fn14 yields additive or even synergistic therapeutic activity. To facilitate clinical translation of TNFR2 targeting on Tregs, we will also develop human TNFR2-specific antibody variants with Fcγ-receptor-independent agonistic activity and various IL-2 receptor targeted TNFR2 agonists. These reagents will be tested and evaluated in a variety of in vitro models but also in knockin mice in which the ectodomain of murine TNFR2 has been replaced by the corresponding domain of human TNFR2.
Prof. Dr. rer. nat. Harald Wajant |
University Hospital Würzburg |
Department of Medicine II |
Molecular Internal Medicine |
Röntgenring 11 |
97070 Würzburg |
T: +49 931 201-71000 |
CSF2+ T cells have been recently described to critically mediate immune-mediated tissue-damage in various autoimmune disease models. Their role within the pathogenesis of GvHD has not been studied yet. Our results indicate that CSF2+ donor T cells critically contribute to intestinal GvHD manifestation suggesting that CSF2 might represent a novel drugable target to limit GvHD. Hence, the overall goal of this research project is to molecularly and functionally define the T cell-extrinsic and -intrinsic signals that drive CSF2+ donor T cell formation and identify the characteristics and effector mechanisms of CSF2+ donor T cells in the immune pathogenesis of GvHD.
Prof. Dr. med. Kai Hildner |
University Hospital Erlangen |
Department of Medicine 1 |
Ulmenweg 18 |
91054 Erlangen |
T: +49 9131 85-35000 o. 85-45173 |
Metabolic deregulation is emerging as a common pathogenic driver of chronic inflammatory as well as fibrosing diseases. We demonstrated in our preliminary resultsthat the expression of ALDH3A2 is deregulated in the skin of cGvHD patients in a TGFb-dependentmanner. Inactivation of ALDH3A2 prevented fibroblast activation, whereas overexpression of ALDH3A2 promoted collagen release and deposition of extracellular matrix. Moreover, inactivation of ALDH3A2 modulated leukocyte infiltration and ameliorated tissue remodelingin the murine cGvHD. With the current project, we aim to study the effects of ALDH3A2 on leukocyteinfiltration and fibroblast activation in experimental cGvHD and to decipher the molecular mechanisms, by which ALDH3A2 regulates inflammation and tissue remodeling in cGvHD.
Prof. Dr. med. Jörg Distler | PD Dr. med. Regina Jischin, PhD |
University Hospital Erlangen | University Hospital Erlangen |
Department of Medicine 3 | Department of Medicine 5 |
Ulmenweg 18 | Ulmenweg 18 |
91054 Erlangen | 91054 Erlangen |
T: +49 9131 85-43008 | T: +49 9131 8543113 |
We previously showed that donor CD4+CD25+Foxp3+ regulatory T cells (Treg) prevent lethal acute GvHD after MHC-mismatched BMT in murine models. We now observed that they also ameliorate ongoing GvHD and the prerequisites for efficacious GvHD therapy are studied in this project. For this purpose, the migration pattern of in vitro expanded donor Treg is examined, their organ-specific T cell receptor (TCR) repertoire selection and their site-specific functional status. Finally, we investigate whether their therapeutic efficacy in GvHD can be enhanced by the overexpression of alloreactive TCRs or tissue-specific homing receptors.
PD Dr. rer. nat. Petra Hoffmann | Prof. Dr. rer. nat. Michael Rehli | Prof. Dr. med. Matthias Edinger |
LIT - Leibniz Institute for Immunotherapy (former RCI) | University Hospital Regensburg | University Hospital Regensburg |
c/o Universitätsklinikum Regensburg | Department of Internal Medicine III | Department of Internal Medicine III |
Franz-Josef-Strauß-Allee 11 | Franz-Josef-Strauß-Allee 11 | Franz-Josef-Strauß-Allee 11 |
93053 Regensburg | 93053 Regensburg | 93053 Regensburg |
T: +49 941 944-38492 | T: +49 941 944-38487 | T: +49 941 944-5582 |
Regulatory T cells (Treg) perform two distinct functions: they maintain self-tolerance and support organ homeostasis by differentiation into specialized tissue Treg cells. We aim to harness the tissue-repair, organ-homeostasis promoting function of tissue-resident Treg cells to prevent or treat graft-versus-host disease after allogeneic bone marrow transplantation. In this respect, we study a TH2-biased tissue Treg population that is present in virtually all organs. We will use loss-of-function and gain-of-function experiments to understand how these cells function during GvHD. Finally, we want to translate these findings into the human context.
Prof. Dr. med. Markus Feuerer |
LIT - Leibniz Institute for Immunotherapy (former RCI) |
c/o Universitätsklinikum Regensburg |
Franz-Josef-Strauß-Allee 11 |
93053 Regensburg |
T: +49 941 944-38121 |
Based on our recent findings on the spatio-temporal kinetics of graft-versus-host disease (GvHD) pathophysiology we discovered a regulatory myeloid cell population that exerts protective functions during the intestinal GvHD effector phase. Employing preclinical mouse models for GvHD and GvL we will interrogate the identity, mechanism of action, and therapeutic potential of this immune-protective myeloid cell subset to improve allo-HSCT.
Prof. Dr. med. Dr. med. univ. Andreas Beilhack | Dr. rer. nat. Mercedes Gomez de Agüero |
Universitätsklinikum Würzburg | Julius-Maximilians-University Würzburg |
Medizinische Klinik und Poliklinik II | Würzburg Institute für Systemimmunologie |
ZEMM Zentrum für Experimentelle Molekulare Medizin | Versbacher Straße 9 |
Zinklesweg 10 | 97078 Würzburg |
97078 Würzburg | T: +49 931 80303 |
T: 0931 201-44040 | |
|
Although experimental data point to a crucial role of antibody mediated damage in chronic GvHD clinical evidence remains restricted to associations but direct proof is lacking. Within the research program we will analyse clonal B cell subpopulations directly involved in clinical chronic GvHD, characterize host specific antibodies including identification of targets and glycosylation profile, analyse T follicular helper cells mediating this process and finally characterize B cell infiltration host-and donor-antibody mediated damage of target organs.
Prof. Dr. rer. nat. Thomas Winkler | Dr. med. Julia Winkler | Prof. Dr. med. Daniel Wolff |
FAU Erlangen-Nürnberg | University Hospital Erlangen | University Hospital Regensburg |
Department of Biology | Department of Medicine 5 | Department of Internal Medicine III |
Nikolaus-Fiebiger-Center for Molecular Medicine | Ulmenweg 18 | Franz-Josef-Strauß-Allee 11 |
Glückstraße 6 | 91054 Erlangen | 93053 Regensburg |
91054 Erlangen | T: +49 9131 85-43112 | T: +49 941 944-5531 |
T: +49 9131 85-29136 | ||
Patients undergoing allogeneic HSCT have an increased risk of cardiovascular disease. To address the interconnection between GvHD and atherosclerosis, we will use a GvHD-atherosclerosis mouse model and analyze GvHD activity, plaque development, as well as local and systemic immune responses. We will further focus on monocytes/macrophages and CD8+ T cells in mediating vascular inflammation and GvHD using relevant knockout mice and/or cell depletion strategies. In addition, we will investigate the potential of immunosuppressive drugs to improve GvHD-related atherosclerosis. It is our aim to define novel approaches to reduce cardiovascular events after HSCT.
Prof. Dr. med. Alma Zernecke-Madsen | Prof. Dr. med. Dr. med. univ. Andreas Beilhack |
University Hospital Würzburg | University Hospital Würzburg |
Department of Experimental Biomedicine II | Department of Medicine II |
Josef-Schneider-Straße 2 | ZEMM Center for Experimental Molecular Medicine |
97080 Würzburg | Zinklesweg 10 |
T: +49 931 201-48331 | 97078 Würzburg |
T: +49 931 201-44040 | |
To understand the underlying mechanisms for the protective effects of vitamin D3 in allogeneic HSCT patients we will investigate vitamin D3-related changes in the microbiome, epithelial barrier function and immune cell infiltration in GvHD target tissues in murine GvHD models. In vitro-analyses will prove direct effects of vitamin D3 on the function of T cells and macrophages. Results will be confirmed in murine models after selective deletion of the vitamin D receptor in T cells, macrophages and epithelial cells. Finally, effector mechanisms will be verified in human GvHD samples. Results should provide the basis for vitamin D3 supplementation of allo-HSCT patients in a prospective clinical trial.
Prof. Dr. rer. nat. Marina Kreutz | PD Dr. rer. nat Heiko Bruns |
University Hospital Regensburg | University Hospital Erlangen |
Department of Internal Medicine III | Department of Medicine 5 |
Franz-Josef-Strauß-Allee 11 | Ulmenweg 18 |
93053 Regensburg | 91052 Erlangen |
T: +49 941 944-5577 | T: +49 9131 85-43163 |
Based on our previous observation, that high enterococcal abundance associates with GvHD, we aim to analyze the causal relationship between enterococci and GvHD. Enterococcal strains collected from GvHD patients will be tested for pathogenicity in epithelial cell cultures as well as in murine models of colitis and GvHD: GvHD will be induced after recolonization of mice pretreated with antibiotics or of germfree mice with apathogenic and highly pathogenic strains. Full sequencing and targeted mutagenesis of specific enterococcal strains will be performed to identify strain specific pathogenicity factors. Finally, the impact of enterocccci on intestinal Treg and IgA reconstitution will be analyzed.
PD Dr. med. Daniela Weber | Prof. Dr. med. Dr. rer. nat. André Gessner | Prof. Dr. med. Ernst Holler |
University Hospital Regensburg | University Hospital Regensburg | University Hospital Regensburg |
Department of Internal Medicine III | Department of Clinical Chemistry and Laboratory Medicine | Department of Internal Medicine III |
Franz-Josef-Strauß-Allee 11 | Franz-Josef-Strauß-Allee 11 | Franz-Josef-Strauß-Allee 11 |
93053 Regensburg | 93053 Regensburg | 93053 Regensburg |
T: +49 941 944-5510 | T: +49 941 944-6401 | T: +49 941 944-5542 |